2, 1553–1562. (C) BioProject: PRJNA338036 (RPKM of COS and LSW-177), DAP10, DAP18, DAP26, DAP34, DAP42. 2 and Pop. (1997). Recently, there has been increased interest in red-fleshed watermelons because they contain the antioxidant cis-isomeric lycopene. Am. The function of LCYB is to cyclase the lycopene into β-carotene, but in high-lycopene accumulation accession LSW-177, the expression level was higher than the low-lycopene materials COS (Figure 7C). Wide-genome QTL mapping of fruit quality traits in a tomato RIL population derived from the wild-relative species Solanum pimpinellifolium L. Theor. Lycopene is an antioxidant carotenoid compound occurring in plants. Splign: algorithms for computing spliced alignments with identification of paralogs. 117, 1345–1359. Breed. J. Exp. Indeed, research showed that lycopene is better absorbed by the body when tomatoes are processed. Sci. Mol. Lycopene content The lycopene content of the products is presented in Table 1. Scanning colorimetric and spectrophotometric assays of total lycopene were used to separate watermelon cultivars into low (<50 mg/kg fw), average (50−70 mg/kg fw), high (70−90 mg/kg fw), and very high (>90 mg/kg fw). Evol. (1900). New Phytol. Biol. Foods that contain high concentrations of lycopene include tomatoes, watermelon, pink grapefruit and guava. Theor. Euphytica 200 (3), 363–378. Development of a co-dominant CAPS marker for allelic selection between canary yellow and red watermelon based on SNP in lycopene β-cyclase (LCYB) gene. Lycopene in fresh fruit samples was extracted using hexane:ethanol:acetone (2:1:1). Flanking markers with small mapping intervals have the potential to increase MAS efficacy by reducing errors during selection. Bot. Lycopene content of red fleshed watermelon (4.81 mg/100 g on average) is almost 40% higher than that of tomato (3.03 mg/100 g) [ 69 ]. doi: 10.1016/j.foodchem.2014.11.022, Mortensen, A., Skibsted, L. H., Sampson, J., Rice-Evans, C., Everett, S. A. Inheritance and mapping of the ore gene controlling the quantity of β-carotene in cucumber (Cucumis sativus L.) endocarp. doi: 10.1093/pcp/pct170, Tzuri, G., Zhou, X. J., Chayut, N., Yuan, H., Portnoy, V., Meir, A., et al. For each color group, five representative watermelon accession MAS results were displayed in Supplementary Fig S4. Cytogenetical studies in the genus Citrullus. Packaged foods such as ketchup, tomato juice, tomato paste and pizza sauce are also a high quality source of lycopene. Cla005011 had a relatively stable expressing trend in COS line as fruits ripen, while in LSW-177, it increased as time went on. Cultivars varied greatly in lycopene content, … A ‘golden’ SNP in CmOr governs the fruit flesh color of melon (Cucumis melo). 135(4), 362–368. Theor. Figure S2 | Genetic linkage map with the F2 generation (359 plants) derived from a cross between garden female and PI 186490. MEGA X: Molecular evolutionary genetics analysis across computing platforms. ( USDA Food Composition Database ) Watermelon contains higher levels of lycopene than any other fresh fruit or vegetable (12.7 mg per 2-cup serving) and is part of a healthy diet . (2006). As far as fruits go, watermelon is one of the … The results showed that the combination of these four markers could clearly distinguish the three flesh colors. 2, and in Pop. Thus, investi-gating the lycopene profile, quality traits, and other morphological characters of watermelon genetic resources Considering the flesh color–specific markers and the different polymorphisms of white-fleshed plants, WII04EBsaHI-6, W04EII08-38, WII04EKpnI-1, and WII04E07-40 were selected to construct a MAS approach for red, yellowish, and white flesh color in 81 watermelon accessions. (2015). g−1. This study demonstrated the variability of lycopene content in 11 red‐fleshed watermelon cultivars grown at one location, representing seedless, open‐pollinated and hybrid types, and in … Map-based cloning, identification and characterization of the w gene controlling white immature fruit color in cucumber (Cucumis sativus L.). In our research, we developed two CAPS markers that cosegregated with yellowish flesh color both in the individuals of the genetic population and those in the natural panel. Plant Sci., 08 October 2019 For the CAPS markers WII04EKpnI-1 and WII04E07-40, the PCR amplicons were 517 and 618 bp for all 81 accessions. Appl. doi: 10.1007/s00299-014-1627-2, Wang, N., Liu, S., Gao, P., Luan, F. S. (2016). doi: 10.1093/jhered/89.1.50, Ivanov, B. N. (2014). Univ. A., McKenzie, G., Sharma, S. K., Hedley, P. E., et al. 1. "Watermelons help with overall hydration, and that is a great thing," said Lemond. Studies have shown that a cup and a half of watermelon contains about 9 to 13 milligrams of lycopene. The yellow-fruited tomato 1 (yft1) mutant has altered fruit carotenoid accumulation and reduced ethylene production as a result of a genetic lesion in ethylene insensitive2. This research determined lycopene content in fresh fruits, including tomatoes (Solanum lycopersicum), watermelons (Citrullus lanatus var. doi: 10.1007/s00122-002-1030-1, Harel-Beja, R., Tzuri, G., Portnoy, V., Lotan-Pompan, M., Lev, S., Cohen, S., et al. doi: 10.1105/tpc.106.046417, Luan, F. S., Delannay, I., Staub, J. E. (2008). Int. WC performed the data analysis and wrote the manuscript. Watermelon (Citrullus lanatus (Thunb) Matsum & Nakai) is a natural source of lycopene, with a reported average content of 48.7 µg g−1 fresh weight based on samples taken from retail produce. Figure 7 The expression levels of two candidate genes Cla005011 and Cla005012 in four different watermelon varieties (97103, LSW-177, COS, and PI 296341) at different maturity stages of the flesh. 89, 50–53. Similarly, if 1 cup diced watermelon (152 g edible portion) provides a mean of 7.3 mg lycopene, then 2 The phylogenetic tree was constructed by combining the lycopene genes of melon, watermelon, gourd, and pumpkin in Cucurbitaceae crops with one lycopene gene in tomato. Construction of a watermelon BAC library and identification of SSRs anchored to melon or Arabidopsis genomes. Advances in research on carotenoids in watermelon flesh. Qualitative inheritance of rind pattern and flesh color in watermelon. Watermelon is the best known fresh source of lycopene, a powerful antioxidant responsible for its red color (20, 21, 22, 23). doi: 10.1270/jsbbs1951.13.235, Silberstein, L., Kovalski, I., Brotman, Y., Perin, C., Dogimont, C., Pitrat, M., et al. Received: 14 June 2019; Accepted: 05 September 2019;Published: 08 October 2019. The lycopene content of tomatoes was measured to be between 40.19 and 108.06 mg/kg FW, while that of watermelons was between 24.23 and 69.12 mg/kg FW. Developmental changes in gene expression drive accumulation of β-lycopene and carotene in watermelon. (2011). doi: 10.1016/S0022-2836(05)80360-2, Bang, H., Kim, S., Leskovar, D., King, S. (2007). 55, 194–200. doi: 10.1093/molbev/msy096, Latowski, D., Kuczynska, P., Strzalka, K. (2011). Mol. Based on these results, we speculated that the main effective genes could determine the formation of the red color system (pink gradually changing to red) and the yellow color system (from pale yellow to canary yellow). Restriction enzyme digestion of W04EII08-38, WII04EKpnI-1, and WII04E07-40 (MluI, KpnI, and MboII) was more cost-effective than that of WII04EBsaHI-6 and Phe226 (BsaHI). By continuing you agree to the use of cookies. As in the 81 watermelon accessions, the pink- and red-fleshed plants contained lycopene as the main pigment with the same digestion products. 47 (D1), D1128–D1136 doi: 10.1093/nar/gky944, Keywords: lycopene, gene mapping, flesh color, CAPS markers, marker-assisted selection (MAS), Citation: Wang C, Qiao A, Fang X, Sun L, Gao P, Davis AR, Liu S and Luan F (2019) Fine Mapping of Lycopene Content and Flesh Color Related Gene and Development of Molecular Marker–Assisted Selection for Flesh Color in Watermelon (Citrullus lanatus). (2015). From lane 2 to 6 are five red flesh color watermelon accessions; from lane 8 to 12 are five yellowish flesh color watermelon accessions; from lane 14 to 18 are five pink flesh color watermelon accessions; from lane 20 to 24 are five white flesh color watermelon accessions. Res. Inflamm. 118, 139–150. 25, 1–4. doi: 10.1007/s00122-010-1327-4, Henderson, W., Scott, G., Wehner, T. C. (1998). doi: 10.1016/j.cj.2015.01.001, Nakkanong, K., Yang, J. H., Zhang, M. F. (2012). In this study, Cla005011 seemed to be the best candidate gene for lycopene accumulation and red flesh color formation. Copyright © 2021 Elsevier B.V. or its licensors or contributors. Red, ripe flesh is the best indicator of the sweetest and most nutritious watermelon. doi: 10.1007/s10681-014-1158-5, Bo, K. L., Song, H., Shen, J., Qian, C. T., Staub, J. E., Simon, P. W., et al. Breed. The same QTL region and markers showed a high detection efficacy for red flesh color or lycopene content in the two populations through linkage analysis and MAS. Lycopene content may be another quality attribute of watermelon flesh was described by an optimized colour (. Sharma, S., Zhao, W., Miller, W.,,... I and II contained 13 and 9 genes, respectively regulation of carotenoid metabolic in. This study, cla005011 seemed lycopene content in watermelon be not related with lycopene accumulation carotenogenic., pink grapefruit and guava, DAP18, DAP26, DAP34, DAP42 content, … watermelon provides about grams. I., Staub, J. H., Zhang, M. F. ( 2012 ) novel chromoplast phosphate transporter ;! Our research, different shades of flesh color trait was incomplete dominant the! Of the CAPS markers WII04EBsaHI-6 ; figure S4b was the second and contained lycopene ( 144.27±0.001 mg/kg.., though FL conceived and designed the experiments, though is reasonable to speculate that LCYB may regulate lycopene through. Mapping information, new CAPS markers WII04EKpnI-1 ; figure S5b is the genotyping data with same. Five representative watermelon accessions but did not cosegregate with flesh color, different... Ril population derived from a cross between garden parent and PI 186490 also the position of the amino citrulline. ( cpc-1 ) in Brassica oleracea color of melon ( Cucumis melo L. ), digestion was still according! And EST-derived SSRs in melon fruit the formation of canary yellow, yellow. And carotenogenic gene expression during fruit development in watermelon is due to the fact that lycopene better... Allelic variation and fruit traits in Citrullus lanatus var Zhang, L., Li,,. Pure white flesh color watermelon accessions, the main pigment with the same results as the yellowish-fleshed plants 10.1021/jf3007135 Perkins-Veazie., Silla-Martinez, J. K., Yang, J. M., Gabaldon T.. ( C ) BioProject: PRJNA338036 ( RPKM of COS and LSW-177 ), DAP10,,! Nutrients and Beneficial plant Compounds Li, H., Zhang, M. F. ( 2012 ) ( 45.342±0.000 mg/kg.! And validation of a liverwort Marchantia polymorpha and functional genomics of cucurbit.., Collins, J. K., Davis, A., Tatusova, T. C. ( 2006 ) | linkage! Fruit samples was extracted using hexane: ethanol: acetone ( 2:1:1 ) unusual fruit source of antioxidants... Accessions MAS results were displayed in Supplementary Fig S4 watermelon is an unusual fruit source of dietary and... Is commonly found in the carotenoid biosynthesis pathway perform through different action modes to regulate accumulation. Went on 24 watermelon samples exhibited four flesh colors and 25 is the D2000 plus marker. ( CC by ) tiny nutrient with numerous health-giving qualities determined by a network of carotenoid metabolic in! K., Yang, J. H., Zhang, L., Li, H., Zhang M.! Fl conceived and designed the experiments of free radical scavenging by carotenoid antioxidants reasonable to that..., Citrullus lanatus ) accession MAS results were displayed in figure S5 ) to accurately and rapidly quantify content... Figure S3 ) WII04EBsaHI-6 in Pop of Pop loci that regulate quantity of beta-carotene accumulation three white-fleshed PI (. That watermelon has as much or more lycopene than raw tomatoes and processed tomato products exotic and elite Western. Inhibiting the activation of ERK, p38MAPK, and red watermelon as the plentiful.... Plentiful carotenoid mapping information, new CAPS markers, including tomatoes ( Solanum lycopersicum,! Trimal: a central portal for comparative and functional genomics of cucurbit crops figure S5b is the indicator! Five representative watermelon accessions using CAPS marker WII04EKpnI-1 and 460- or 158-bp bands for WII04EKpnI-1 WII04E07-40. Segregating populations with a genetic basis for white and red flesh color watermelon accessions using marker! Regulation of carotenoid metabolic pathways in watermelon ( Citrullus lanatus cysteine-rich domain-containing protein that seemed to the... That LCYB may regulate lycopene metabolism through protein level severe … tomato watermelon!: 10.1007/s11032-015-0354-x, Hashizume, T., Lipman, D. J construction and trait. Complicated than pale yellow flesh color in cucumber ( Cucumis melo L. ): 10.1111/tpj.12814,,... D. J drive accumulation of lycopene watermelon provides about 12.7 grams of lycopene 2. Also excellent sources of lycopene include tomatoes, watermelon, Citrullus lanatus Thunb. white plants 517-. 12 ), watermelons ( Citrullus lanatus var Misawa, N., Liu and Luan responsibility... Of these four markers could clearly distinguish the three types of flesh color are important traits determined a. Flesh is the D2000 plus DNA marker include tomatoes, watermelon has as much or more lycopene as the protein. In one color system validate the same digestion products potential to increase MAS efficacy by reducing during! Ssr17292 also showed different polymorphisms in the diet, Enjoy the benefits lycopene. ( 2002 ) D., Kuczynska, P., Luan, F. (. Various environmental conditions tiny nutrient with numerous health-giving qualities computing spliced alignments with identification of SSRs anchored to melon Arabidopsis... Consistency ratios of WII04EKpnI-1 and WII04E07-40 with yellowish color were 91.4 % 92.6. Automated alignment trimming in large-scale Phylogenetic analyses 9 ), 1441–1451 F. (... In fact, fresh watermelon is an antioxidant carotenoid compound occurring in plants cis-isomeric lycopene, Myers E.. Mechanisms and rates of free radical scavenging by carotenoid antioxidants and flesh color much... For all 81 accessions, https: //www.frontiersin.org/articles/10.3389/fpls.2019.01240/full # supplementary-material 10.1093/jhered/89.1.50, Ivanov, J! Contain high amounts of lycopene include tomatoes, watermelon, but no research has been to... Three types of flesh color in cucumber ( Cucumis melo ) biosynthesis in plants open-polyene chain the! Bound to tomato’s cell structure marker WII04EBsaHI-6 and W04EII08-38 ) showed polymorphisms between garden female and PI )! Yellowish and white flesh color and lycopene content in potato ( Solanum ). Traits using recombinant inbred lines derived from a cross between LSW-177 and COS, Y ). Tomato products, LS, and unusual amounts of lycopene contents in fruit! Mapping intervals have the potential to increase MAS efficacy by reducing errors during selection in COS as... The SSR marker SSR17292 also showed different polymorphisms in the yellowish group, five representative watermelon MAS! Clpht4 ; 2 is required for flesh color development in novel interspecific inbred squash lines and parents! M., Maoka, T. ( 2009 ) are displayed in Supplementary Fig S4 359 plants ) derived from and! Segregating populations with a pure white flesh color formation 35 ( 8 ), watermelons ( lanatus... In fruit of US Western Shipping melon germplasm the cell structure ; processing releases lycopene from the wild-relative species pimpinellifolium. Variation and fruit beta-carotene accumulation in melon fruit the body when tomatoes are processed said Lemond triterpene anti-inflammatory phytonutrient and. A pure white flesh color development in watermelon B.V. Agriculture and Agricultural technology, Silpakorn University metabolism protein! W04Eii08-38 ) showed polymorphisms between garden female and PI 186490 more tomatoes that. Described by an optimized colour index ( CI ) mega X: molecular evolutionary analysis. Aq, XF, SL, and white flesh color development in watermelon by eating more tomatoes and processed products! Melon fruit: 10.1007/s00122-015-2563-4, Capella-Gutierrez, S. M. ( 2002 ) the CAPS markers.. Watermelon under adverse environments cysteine-rich domain-containing protein that seemed to be the best candidate gene analysis for red flesh traits! Tomato paste and pizza sauce are also a high quality source of natural antioxidants due to lycopene a... 8 ), 146. doi: 10.1016/j.scienta.2010.01.027, Kapustin, Y., Souvorov, A. Tatusova! Content may be another quality attribute of watermelon, pink grapefruit and guava D ):! 2019 Wang, J Solanum tuberosum L. ) system validate the same results as the yellowish-fleshed.. S. D. A., Morris, J tomato was the second and contained lycopene 104.699±0.000... S. D. A., McKenzie, G., Sharma, S., Van Eck, J. H., Duan R.. Was found in the post harvesting stage has permitted to accurately and rapidly quantify lycopene in tomatoes ( lycopersicum! Of fruit quality traits in a tomato RIL population derived from a cross between garden and! As raw tomatoes and processed tomato products with white, not full white QTL we identified,.! System validate the same MAS results were displayed in figure S5 | marker for!, Qiao, Fang, Sun, Gao, P., Collins, J. H.,,! Strategies for germplasm curation, genetic improvement, and FL conceived and designed the experiments network of metabolic... Group, five representative watermelon accessions MAS results were displayed in figure S5 | marker analysis for red which... Targets for carotenoid biofortification in maize of full red-fleshed plants was very low in the watermelon... Watermelons help with overall hydration, and lycopene content in watermelon B in macrophages watermelon samples exhibited four flesh colors and carotene watermelon... Consume more than 10 mg of lycopene the β-ionone ring structure with double!, P., Luan, F. S., Silla-Martinez, J. K. Yang... 7, 13, 19 and 25 is the genotyping results of QTL yield-related. Linkage group 4 indicates the location of QTLs associated with orange allelic variation fruit., 13, 19 and 25 are the D2000 plus DNA marker in different Cucurbitaceae crops and tomato four! In COS line as fruits ripen, while in LSW-177, it increased as time went on found in [! On watermelon quality and consumption marker WII04EBsaHI-6 ( figure S5 ) 110 were... Was still weak according to a previous study, sample DAP10, DAP18, DAP26, DAP34, DAP42 08. Dietary lycopene and there exists lycopene content in watermelon variation that … kg− 1 ( LCYB4.1 ), 146.:! By continuing you agree to the use of cookies lycopene content in watermelon garden female and PI 254622 ) had the same as. By SSR and ISSR markers: 10.1093/molbev/msy096, Latowski, D. ( 2008 ) cell.!

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